Sphingosomes Development Services

Sphingosomes are clinically used delivery systems for chemotherapeutic agents, biomacromolecules, and diagnostic agents. Due to flexibility in size and composition, different types of sphingosomes have been developed.

Protheragen-ING specializes in the development of sphingosomes Drug Delivery Systems (DDS) to revolutionize targeted drug delivery. Our team of experienced scientists and researchers are at the forefront of sphingosomes DDS development, utilizing cutting-edge technologies and industry best practices to deliver innovative solutions for drug delivery challenges.

Sphingosomes as DDS

Sphingosomes are amphiphilic concentric vesicular delivery systems formed from a sphingolipid bilayer that surrounds an aqueous volume and has an acidic internal pH. These vesicles comprise sphingolipids (SL) and cholesterol (Chol) in molar ratios ranging from 75:25 to 30:50, with 55:45 SL/Chol being optimal.

Sphingosomes are advanced DDSs that harness the power of sphingolipids, naturally occurring lipids found in cell membranes, to encapsulate and deliver drugs to specific sites in the body.

Fig.1 Solubilization of free β-sitosterol in milk sphingomyelin and polar lipid vesicles as carriers.Fig. 1 Solubilization of free β-sitosterol in milk sphingomyelin and polar lipid vesicles as carriers. (Lopez C, et al. 2023)

By leveraging the unique properties of sphingolipids, Sphingosomes DDS offer numerous advantages over traditional drug delivery methods, including:

Our Expertise

We offer comprehensive sphingosomes DDS development services tailored to meet the specific needs of our clients. Our multidisciplinary team possesses extensive knowledge and expertise in areas such as:

We excel in designing and optimizing sphingosomes formulations to encapsulate a wide range of drugs, including small molecules, peptides, proteins, and nucleic acids. Our formulation experts employ state-of-the-art techniques to ensure optimal drug loading, stability, and release characteristics.

We employ a variety of advanced analytical techniques to characterize and evaluate the physicochemical properties of sphingosomes DDS. This includes particle size analysis, morphology assessment, drug encapsulation efficiency, drug release kinetics, and stability studies.

Our team specializes in developing strategies to achieve targeted drug delivery using sphingosomes DDS. We can incorporate ligands, antibodies, or peptides onto the surface of sphingosomes to enhance their affinity for specific cell types or tissues, thereby increasing drug accumulation at the desired site.

We conduct rigorous in vitro and in vivo studies to assess the performance and efficacy of sphingosomes DDS. Our scientists utilize cell-based assays, animal models, and imaging techniques to evaluate drug release profiles, cellular uptake, tissue distribution, pharmacokinetics, and therapeutic outcomes.

Protheragen-ING's Technical Approach

Preparation of Sphingosomes

Fig.2 PE-loaded sphingosome was prepared by an improved thin film hydration method.Fig. 2 PE-loaded sphingosome was prepared by an improved thin film hydration method (lipid hydration method). (AlMadalli HJ, et al. 2024)

Reduce the size of Sphingosomes

Application of Sphingosomes as Carrier

Sphingosomes can be used as vectors to treat proliferative diseases, immune diseases, infectious diseases, vascular diseases, rheumatoid diseases, and inflammatory diseases.

Representative drugs include prostaglandins, amphotericin B, methotrexate, cisplatin, vincristine, vinblastine, doxorubicin, camptothecin, ciprofloxacin, progesterone, testosterone, estradiol, Clomethasone and steroids such as vitamin E esters and dexamethasone.

Are you ready to unlock the full potential of Sphingosomes DDS for your drug delivery needs? Contact us today to discuss your project requirements, explore collaboration opportunities, and embark on a journey toward advanced targeted drug delivery.


  1. Lopez C, et al. (2023). "Solubilization of Free β-sitosterol in Milk Sphingomyelin and Polar Lipid Vesicles as Carriers: Structural Characterization of The Membranes and Sphingosome Morphology." Food Research International, 165, 112496.
  2. AlMadalli HJ, et al. (2024). "Pomegranate Extract-loaded Sphingosomes for The Treatment of Cancer: Phytochemical Investigations, Formulation, and Antitumor Activity Evaluation." PLoS One, 19(2), e0293115.


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